Researchers develop test to detect early onset of heart attacks

10 Jan 2014 iopp"> iopp

A new “fluid biopsy” technique that could identify patients at very high risk of a heart attack by identifying specific cells as markers in the bloodstream has been developed by a group of researchers in the US.

The technique, which has been described today, 10 January, in IOP Publishing’s journal Physical Biology, works by identifying circulating endothelial cells (CECs) and has been successful in distinguishing patients undergoing treatment for a recent heart attack with a healthy control group.

The researchers, based at The Scripps Research Institute in California, believe the technique can now be tested on patients who exhibit symptoms but are yet to experience a heart attack, and believe it is a genuine candidate for clinical use, of which there is no predictive test—at least not of satisfying accuracy—currently available.

Co-author of the study Professor Peter Kuhn said: “The goal of this paper was to establish evidence that these circulating endothelial cells can be detected reliably in patients following a heart attack and do not exist in healthy controls, which we have achieved.”

“Our results were so significant relative to the healthy controls that the obvious next step is to assess the usefulness of the test in identifying patients during the early stages of a heart attack.”

Endothelial cells, which line the walls of the arteries, have been strongly linked to on-going heart attacks when circulating in the bloodstream and are thought to arrive there when diseased plaque builds up, ruptures and ulcerates, causing inflammation in the arteries.

This damage can cause blood clots to form which prevent blood from flowing through the arteries and ultimately results in a heart attack.

As such, the researchers developed a procedure called the High-Definition Circulating Endothelial Cell (HD-CEC) assay to detect and characterise CECs in the blood samples of 79 patients who had experienced a heart attack at the time of sampling.

For comparison they also used the assay on two control groups, which consisted of 25 healthy patients and seven patients undergoing treatment for vascular disease.

The assay was able to identify CECs by their morphological features and their reactions with specific antibodies. The cells were shown to be significantly elevated in the heart attack patients compared to the healthy controls and were detected with high sensitivity and high specificity.

The researchers also compared their results with a commercially available test, called CellSearch®, which has previously been approved by the US Food and Drug Administration (FDA) to enumerate circulating tumour cells in cancer patients.   

The HD-CEC test showed a higher specificity for CECs compared to CellSearch® because it used a direct analysis method and was free of bias from an enrichment stage.

“Our assay effectively analyses millions of cells, which is more work but guarantees that you are analysing all of the potential cells. With the enrichment stage in the CellSearch® methodology it is possible that the important cells you wish to study could be lost,” continued Professor Kuhn.